4th European Virology Congress.
4th European Virology Congress, Congress Center “Villa Erba”, Cernobbio, Como, April 7 – 11, 2010.
A.De Montis1, C.Lauterio1, C.Passotti1, C.Frongia2, M.Pittau2, Michela Piga3, A.Cerina4, D.Casimiri5, M.G.Schirru5, P.L. Schirru5.
1Research and Development Laboratory bcs Biotech, 2 HPV Cloning Laboratory bcs Biotech, 3Pathological Anatomy SS Trinità ASL-8, 4 Obstetrics and Gynecology Department Policlinico Città di Quartu, 5Central Laboratory Policlinico Città di Quartu, Cagliari – Italy
Background. Diagnostic techniques should be conformed to the HPV infection state of art and its role on the cervix cancer etiopathogenesis. Our effort is to investigate the E6/E7 relevance as a further marker of HPV infection by commercial kit. Methods. 166 women cervical spatula samples were evaluated by new biochip diagnostic test for the detection of two HPV genomic sequences: L1, 19 types including 15 oncogenic high-medium risk (HR) types, and E6/E7, five genetic groups comprising high-medium risk types. Results. Samples tested were: 6 invalids, 84 negative, 60 positive for L1 typable HPV, and 16 positive only for E6/E7 sequences. Among positive samples for typable HR virus, HPV-16 genotype was the most frequent, followed by HPV-35, HPV-58 and HPV-18. The most frequent low risk type was HPV-6/11, within HPV-6/11 cases 4 showed E6/E7 sequences of non typable HR HPV. The histological analysis of positive women only for E6/E7 and for HPV6/11+ E6/E7, showed that 14 of 20 were CIN1, and 6 of 20 demonstred lesion of grade 2. DNA sequencing of the E6/E7 confirmed that these women were infected by common HR type (no easy detectable cause low viral load and/or physical state of HPV) or by not very common type (HPV-26, 30, 53, 54, 61, 67). Conclusions. We can conclude that, even if we have a small sample, the data highlighted the importance of seeking also genomic E6/E7 in order to reduce the number of false negatives and to exclude the persistence of undetectable or underdiagnosed HR virus.